Hot selling high quality proteinase k with reasonable price CAS 39450-01-6

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Hot selling high quality  proteinase k with reasonable price CAS 39450-01-6
Hot selling high quality  proteinase k with reasonable price CAS 39450-01-6
Hot selling high quality  proteinase k with reasonable price CAS 39450-01-6
Hot selling high quality  proteinase k with reasonable price CAS 39450-01-6
Hot selling high quality  proteinase k with reasonable price CAS 39450-01-6
Hot selling high quality  proteinase k with reasonable price CAS 39450-01-6
Hot selling high quality  proteinase k with reasonable price CAS 39450-01-6

Hot selling high quality proteinase k with reasonable price CAS 39450-01-6

  • Item No.TOP
    MOQ25 Milligram
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From $5.002.00 / Milligram
$125.00
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If over 500pcs, mass production will start until pre-production sample has been approved.

Est. 7 days production

Quick Details
Product name Proteinase K
Appearance White Powder
Place of Origin CN;SHN
Usage Animal Pharmaceuticals
Brand Name Xigema
certificateISO
Model Number TOP
Application Pharmaceutical Raw Intermediates
CAS39450-01-6
Color White Color
Name Proteinase K
Assay HPLC 99%
Shelf Life 2 Years
Grade Standard Medicine Grade
Type Gastrointestinal Agents
CAS No. 39450-01-6
Other Names Proteinase K
MFN/A
EINECS No. 254-457-8
Purity99%min
Grade Phamaceutical Grade

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Selling Units:
Milligram
Product Description

company

 

Prodcut Description.png

 

 CAS No.: 39450-01-6
EINECS No.: 3.4.21.64

Form: Lyophilized White Powder.
Source: Mutated gene from Tritirachium limber, Expressed in recombinant yeast.
Specific Activity: ≥34U/mg dry weights
Package: 100mg/Bottle, 1g/Bottle (Standard) or 1-200 gram Customer packing.
Storage Conditions:Store at 4 to -20ºC recommended.
Mutated Proteinase K powder is stable at room temperature in 3 years.
Expiration : Three years if kept below 4ºC.
Molecular Weight: 29,300 D
QC Assays:
Activity Assay and Unit Definitions: 
 One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37º C, pH7.5.
DNase Activity: none detectable enzyme activity with λ DNA after 6 hours incubation at 37ºC.
RNase Activity: none detectable of ribnuclease activity after 16 hours incubation at 25 º C
Protein Purity: over 99% (Native-PAGE and SDS-PAGE assay)

SIGMA-COA .png

 

Description

White  powder

Complies

Identification

IR

Complies

 

HPLC

Complies 

Heavy metal

≤10ppm

5ppm

Pb

≤3ppm

1.5ppm

Hg

≤0.1ppm

0.05ppm

Cd

≤1ppm

0.2ppm

Loss on drying

≤0.5%

0.12

Residue on lgnition

≤0.1%

0.03

Single impurity

≤0.5%

0.12

Total impurity

≤1.0%

0.29

Total bacteria

≤1000cfu/g

<1000

Yeast and Mould

≤100 cfu/g

<000

E.coli/25g

Absent

Absent

Salmonella/25g

Absent

Absent

Assay

≥99.0%

99.4%

Conclusion

Conforms with USP/EP standards

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The application of this Mutated Proteinase K is similar with wild type Proteinase K. But the mutated one has higher specific activity and more stable at RT. It is a broad substrate non-specific serine Proteinase. It is active over the pH range 4-11. It was used on any situation to digest native and denatured proteins. For instance, it is used for isolating mRNA or genomic DNA from different tissues and modifying glycoprotein for structure studies.Mutated Proteinase K is active with SDS, urea and EDTA and active between the temperature15-75ºC.

 

Now,Proteinase K is a broad substrate non-specific serine proteinase. It was used on isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from different kinds of cells.

It’s been used on glycoprotein modification and protein structure studies also. Proteinase K is active with SDS, urea and EDTA.


Proteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.
Adding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high molecular DNA or RNA. A variety of methods have been established, which are documented in numerous publications.
Recently, Proteinase K has been used for the detection of BSE forming proteins which are uniquely resistant towards the enzyme's proteolytic cleavage.


Proteinase K is very useful in the analysis of membrane structure by means of modification of proteins and glycoproteins on cell surfaces.


Because of the cleavage specificity Proteinase K, characteristic fragments of proteins are obtained which are helpful in revealing the structure and function of proteins, particularly enzymes.

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FAQ

 

Q1: Are you a manufacturer?
Answer: Yes, we are factory founded on 2002.

Q2: How to contact with us?
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Q3:Which kind of payment terms do you accept?
For small order,you can pay by T/T,Western Union or Paypal,nomal order by T/T to our company account

Q4:Can you give me a discount price?
Surely,It depend on your qty

Q5:How can i get a sample?
free samples is available,but freight charges will be at your account and the charges will be return to you or deduct from your order in the future.

Q6: How to confirm the Product Quality before placing orders?
A:You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

Q7:How do you treat quality complaint?
A:First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.

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